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capturem his tagged purification miniprep kit  (TaKaRa)


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    Structured Review

    TaKaRa capturem his tagged purification miniprep kit
    Capturem His Tagged Purification Miniprep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/purification+miniprep+kit/Capturem+His-Tagged+Purification+Miniprep+Kit/pm42043053-263-13-19
    Average 99 stars, based on 70 article reviews
    capturem his tagged purification miniprep kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sonication:

    Article Title: Biotransformation of zearalenone to non-estrogenic compounds with two novel recombinant lactonases from Gliocladium.
    Article Snippet: .. The 6×His-tagged proteins were extracted by sonication in PBS (pH 7.4) containing 1 mM phenylmethanesulfonyl fluoride and 1 mM dithiothreitol, purified by a CapturemTM His-Tagged Purification Miniprep Kit (TAKARA, USA), and desalted with a Pur-A-LyzerTM Midi Dialysis Kit (3.5 kDa) according to the manufacturer’s instructions. .. The concentration of proteins was analysed using a Bradford Protein Assay Kit (TAKARA, USA) with bovine serum albumin as the standard.

    Article Title: Biotransformation of zearalenone to non-estrogenic compounds with two novel recombinant lactonases from Gliocladium
    Article Snippet: .. The 6×His-tagged proteins were extracted by sonication in PBS (pH 7.4) containing 1 mM phenylmethanesulfonyl fluoride and 1 mM dithiothreitol, purified by a CapturemTM His-Tagged Purification Miniprep Kit (TAKARA, USA), and desalted with a Pur-A-LyzerTM Midi Dialysis Kit (3.5 kDa) according to the manufacturer’s instructions. .. The concentration of proteins was analysed using a Bradford Protein Assay Kit (TAKARA, USA) with bovine serum albumin as the standard.

    Purification:

    Article Title: Biotransformation of zearalenone to non-estrogenic compounds with two novel recombinant lactonases from Gliocladium.
    Article Snippet: .. The 6×His-tagged proteins were extracted by sonication in PBS (pH 7.4) containing 1 mM phenylmethanesulfonyl fluoride and 1 mM dithiothreitol, purified by a CapturemTM His-Tagged Purification Miniprep Kit (TAKARA, USA), and desalted with a Pur-A-LyzerTM Midi Dialysis Kit (3.5 kDa) according to the manufacturer’s instructions. .. The concentration of proteins was analysed using a Bradford Protein Assay Kit (TAKARA, USA) with bovine serum albumin as the standard.

    Article Title: Production method for L-cyclic amino acids
    Article Snippet: .. The thus obtained soluble protein fraction was purified using His-Tagged Purification Miniprep Kit (manufactured by Clontech Laboratories, Inc.), to obtain MaALD1. ..

    Article Title: Gene isolation and enzymatic characterization of UDP-glycosyltransferases of terpene glucoside biosynthesis involved in linalyl-glycoside accumulation in Coffea arabica.
    Article Snippet: Terpenes, one of the secondary metabolites produced by plants, have diverse physiological functions.. They are volatile compounds with physiological bioactivities (e.g., insect repellent, attracting enemies, and interacting with other plants).. Terpenoids are also essential for flavor and aroma in plant-derived foods.

    Article Title: Adding value to banana farming: Antibody production in post-harvest leaves.
    Article Snippet: Banana, a globally popular fruit, is widely cultivated in tropical and sub-tropical regions.. After fruit harvest, remaining banana plant materials are low-value byproducts, mostly composted or used as fibre or for food packaging.. As an aim to potentially increase farmer income, this study explored underutilised banana biomass as a novel plant tissue for production of a high-value product.

    Article Title: Identification and functional analysis of insulin-like 3 during gonad development in the hermaphroditic orange-spotted grouper, Epinephelus coioides
    Article Snippet: The relaxin family, a group of peptides, is now considered as a major player in the reproductive processes in teleosts fishes.. Insulin-like peptide 3 (INSL3) is a core relaxin, plays a key role in testis development and male fertility.. However, its regulatory mechanism remains unclear.

    Article Title: Biotransformation of zearalenone to non-estrogenic compounds with two novel recombinant lactonases from Gliocladium
    Article Snippet: .. The 6×His-tagged proteins were extracted by sonication in PBS (pH 7.4) containing 1 mM phenylmethanesulfonyl fluoride and 1 mM dithiothreitol, purified by a CapturemTM His-Tagged Purification Miniprep Kit (TAKARA, USA), and desalted with a Pur-A-LyzerTM Midi Dialysis Kit (3.5 kDa) according to the manufacturer’s instructions. .. The concentration of proteins was analysed using a Bradford Protein Assay Kit (TAKARA, USA) with bovine serum albumin as the standard.

    Article Title: Post-ingestive stability of a mulberry Kunitz-type protease inhibitor MnKTI-1 in the digestive lumen of silkworm: dual inhibition towards α-amylase and serine protease.
    Article Snippet: BACKGROUND: Adaptation of specialist insects to their host plants and defense responses of plants to phytophagous insects have been extensively recognized while the dynamic interaction between these two events has been largely underestimated.. Here, we provide evidence for characterization of an unrevealed dynamic interaction mode of digestive enzymes of specialist insect silkworm and inhibitor of its host plant mulberry tree.. RESULTS: MnKTI-1, a mulberry Kunitz-type protease inhibitor, whosemessenger RNA (mRNA) transcription and protein expression in mulberry leaf were severely triggered and up-regulated by tens of times in a matter of hours in response to silkworm, Bombyx mori, and other mulberry pest insects, suggesting a quick response and broad spectrum to insect herbivory.

    Article Title: E3 ligase Praja1 mediates ubiquitination and degradation of microtubule-associated protein Tau
    Article Snippet: .. A pull-down assay was performed using a Capturem His-Tagged Purification Miniprep Kit (#635710, Takara, Shiga, Japan). ..

    Recombinant:

    Article Title: Post-ingestive stability of a mulberry Kunitz-type protease inhibitor MnKTI-1 in the digestive lumen of silkworm: dual inhibition towards α-amylase and serine protease.
    Article Snippet: BACKGROUND: Adaptation of specialist insects to their host plants and defense responses of plants to phytophagous insects have been extensively recognized while the dynamic interaction between these two events has been largely underestimated.. Here, we provide evidence for characterization of an unrevealed dynamic interaction mode of digestive enzymes of specialist insect silkworm and inhibitor of its host plant mulberry tree.. RESULTS: MnKTI-1, a mulberry Kunitz-type protease inhibitor, whosemessenger RNA (mRNA) transcription and protein expression in mulberry leaf were severely triggered and up-regulated by tens of times in a matter of hours in response to silkworm, Bombyx mori, and other mulberry pest insects, suggesting a quick response and broad spectrum to insect herbivory.

    Pull Down Assay:

    Article Title: E3 ligase Praja1 mediates ubiquitination and degradation of microtubule-associated protein Tau
    Article Snippet: .. A pull-down assay was performed using a Capturem His-Tagged Purification Miniprep Kit (#635710, Takara, Shiga, Japan). ..



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